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Objective To understand the sleep quality and psychological status of officers and soldiers in naval force stationed in Hubei, China during the coronavirus disease 2019 (COVID-19) epidemic, and to explore the influencing factors, so as to maintain the physical and mental health of officers and soldiers during the epidemic. Methods Pittsburgh sleep quality index (PSQI) scale, patient health questionnaire-9 (PHQ-9), generalized anxiety disorder-7 (GAD-7) and impact of event scale-revised (IES-R) were used to evaluate the sleep and psychological status of officers and soldiers in a naval force stationed in Hubei province. Results A total of 238 questionnaires were sent out, and 232 valid questionnaires were collected, with an effective rate of 97.5%. The median score of PSQI scale was 2.00 (1.00, 5.00), and poor and very poor sleep qualities accounted for 5.6% (13/232) and 0.9% (2/232), respectively. The median score of PHQ-9 was 0.00 (0.00, 2.00), and mild, moderate and severe depression accounted for 12.1% (28/232), 0.9% (2/232) and 0.4% (1/232), respectively. The median score of GAD-7 was 0.00 (0.00, 0.00), and mild anxiety was found in 7.8% (18/232) participants. The median score of IES-R was 1.00 (0.00, 5.00), and mild and moderate psychological stress accounted for 12.5% (29/232) and 1.3% (3/232), respectively. Conclusion The sleep quality and psychological status of the officers and soldiers from the naval force stationed in Hubei are generally good during the COVID-19 epidemic. A small proportion of them have mild psychosomatic disorders, such as poor sleep quality, depression, anxiety and psychological stress reaction. Psychological counseling and humanistic care should be given to better maintain the physical and mental health of naval forces and ensure their combat capability.
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Objective: To overall evaluate the mutual detoxication mechanism of Tripterygii Radix et Rhizoma(LGT) compatible with Lysimachiae Herba(JQC) in tumor-bearing state.Method: Twelve differentially characteristic components before and after compatibility were used as chemical composition spectrum,six indicators including serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),creatinine(Cr) and urea nitrogen(BUN),malondialdehyde(MDA) levels in the liver and kidney tissues were used as attenuation spectrum,and twelve biological indicators including glutathione(GSH),glutathione-S-transferase(GST),glutathione peroxidase(GPx),superoxide dismutase(SOD),catalase(CAT) and interleukin(IL)-10 in the liver and kidney were used as the biological information spectrum.Mutual detoxication mechanisms of LGT compatible with JQC in tumor-bearing state were overall evaluated by principal component analysis(PCA),and the contribution of chemical components and biological indicators to mutual detoxication was further evaluated by gray correlation analysis(GCA) of "chemical composition spectrum-attenuation spectrum-biological information spectrum".Result: Compared with the model group,the attenuation spectrum scores Z values of S180(Z1 value) and H22(Z3 value) increased significantly after LGT being used alone(PZ1 value and Z3 value caused by LGT when the ratio of LGT and JQC was 4:1,2:1,1:1,1:2 and 1:4(PZ values(Z1 value and Z3 value) of LGT-JQC in the mass ratios including 4:1,1:1,1:2 and 1:4 was significantly higher than that in the ratio of 2:1(PZ values of the bioinformatics scores in the S180(Z2 value) and H22(Z4 value) tumor-bearing state,these two values were significantly increased after compatibility with JQC.The chemical components contributing the most to the attenuating effect of S180 and H22 in tumor-bearing state were 3# and 10#,respectively.The most important biological indicators were kidney GPx and renal GSH.Conclusion: LGT combined with JQC in the mass ratio of 4:1-1:4 can attenuate LGT-induced subacute toxicity in S180 and H22 tumor-bearing state,and the best ratio of such effect is 2:1.The attenuating effect reflects the thought of "there is no reason why there is no meteorology".The mechanism of attenuating action involves antioxidative damage and anti-inflammatory reaction of the liver and kidney,especially the renal GPx(S180) and renal GSH(H22) as the greatest contribution to the detoxication mechanism.
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Apoptosis is an important feature of atherosclerotic plaques, and it has both beneficial and detrimental effects depending on the cell type and plaque stage.Since apoptotic cells in advanced plaques launch proatherogenic inflammatory responses,efferocytosis is available to withstand atherosclerosis progression. Several efferocytosis sys-tems,composed of different phagocytic receptors,apoptotic ligands,and bridging molecules,can be identified.Im-paired efferocytosis pathway causes an overdue clearance of apoptotic cells and promotes secondary necrosis and the formation of necrotic core which leads to the instability and even rupture of plaques.
ABSTRACT
<p><b>OBJECTIVE</b>The pathogenic mechanisms of chronic lung disease (CLD) of premature infants are not fully understood. Its final pathological changes have been shown to relate with lung cell proliferation. The aim of the study was to explore the relationship of the expression of cyclin dependent kinase 4 (CDK4) mRNA and cyclin dependent kinase inhibitor (CDKI) p21 mRNA with lung cell proliferation.</p><p><b>METHODS</b>Eighty premature rats were randomly assigned to a hyperoxia group and a control group (n=40 each). The hyperoxia group was exposed to high concentration of oxygen (FiO2 >0.90 and developed CLD. The control group was exposed to room air (FiO2 =0.21). The rats were randomly subdivided into groups sacrificed at 1, 3, 7, 14 and 21 days of exposure. Lung tissues were collected and made into 5 microm sections. Expression of proliferating cell nuclear antigen (PCNA) in lung tissues was detected by immunohistochemistry. Dynamic expression of CDK4 mRNA and p21 mRNA were detected by in situ hybridization. Lung histomorphology and fibrosis were observed by microscopy.</p><p><b>RESULTS</b>Expression of PCNA and CDK4 mRNA of lung cells in the hyperoxia group increased significantly after 7 days of exposure, and further increased after 14 and 21 days compared with controls. p21 mRNA expression in the hyperoxia group significantly increased after 1 and 3 days of exposure. After 7 days, p21 mRNA expression progressively decreased, but remained at a higher level than control through 21 days of exposure. PCNA expression was positively correlated to CDK4 mRNA expression (r=0.83, P < 0.05) and negatively correlated to p21 mRNA expression (r=-0.81, P < 0.05) in the hyperoxia group between 7 and 21 days of exposure.</p><p><b>CONCLUSIONS</b>Hyperoxia can induce lung cell proliferation in premature rats. The over-expression of CDK4 gene and the decreased expression of p21 gene in lung tissues may be associated with lung cell proliferation induced by hyperoxia.</p>